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- 英文名：
TotalSeq™-C0960 APC Streptavidin
- 供应商：
北京云肽生物科技有限公司
BioLegend 405157 TotalSeq™-C0960 APC Streptavidin
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 405157 TotalSeq™-C0960 APC Streptavidin
产品简介：
产品品牌：BioLegend
产品货号：405157
产品名称：BioLegend 405157 TotalSeq™-C0960 APC Streptavidin
产品规格：10 µg
产品说明：
Product Details
Verified Reactivity Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation
Streptavidin is conjugated with the fluorophore and the TotalSeq™ oligomer under optimal conditions.
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application PG - Quality tested
Recommended Usage
This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the fluorophore or oligomer conjugation. For cell staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For applications related to barcoded MHC tetramers, please follow our Flex-T™ protocol. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the streptavidin dilution (0.06 µg of streptavidin in 100 µL of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
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BioLegend 325228 Brilliant Violet 421™ anti-human CD337 (NKp30) P30-15 100 tests
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BioLegend 325506 PE anti-human IgE MHE-18 100 tests
BioLegend 325508 APC anti-human IgE MHE-18 100 tests
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BioLegend 325607 APC anti-human CD14 HCD14 25 tests
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BioLegend 325612 Alexa Fluor® 647 anti-human CD14 HCD14 100 tests
BioLegend 325614 Alexa Fluor® 700 anti-human CD14 HCD14 100 ug
BioLegend 325617 PE/Cyanine7 anti-human CD14 HCD14 25 tests
BioLegend 325621 PerCP/Cyanine5.5 anti-human CD14 HCD14 25 tests
BioLegend 325622 PerCP/Cyanine5.5 anti-human CD14 HCD14 100 tests
BioLegend 325627 Brilliant Violet 421™ anti-human CD14 HCD14 25 tests
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文献和实验相关实验
式染色带来的影响我们才能更好的做好流式实验。固定对荧光染料的影响是什么？主要表现在固定剂对串联染料（tandem-dye，如 APC/Cy7、PE/Cy7 等）的影响：一个是固定剂有可能造成串联染料的断裂，比如 APC-Cy7 和 4%PFA 接触时间超过 4 个小时染料就有可能断裂，所以如果细胞直接重悬在固定液里面建议尽快上机，如果要放到第二天上机，需离心去除固定液后重悬在流式染色液或者是 PBS 里面，当然有一些新的串联荧光素可以有效减少这种情况，比如 BioLegend 的 APC/Fire750TM
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
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BioLegend 405157 TotalSeq™-C0960 APC Streptavidin
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