BioLegend 394633 TotalSeq™-B02

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BioLegend 394633 TotalSeq™-B0252 anti-human Hashtag 2
产品简介：
产品品牌：BioLegend
产品货号：394633
产品名称：BioLegend 394633 TotalSeq™-B0252 anti-human Hashtag 2
产品规格：10 µg

产品说明：
Clone   LNH-94; 2M2;
Regulatory Status   RUO
Other Names
Na+/K+ ATPase β3, ATP1B3, β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype   Mouse IgG1, κ (all clones)
Barcode Sequence    TGATGGCCTATTGGG

描述
TotalSeq™抗人Hashtag试剂旨在标记大多数人类细胞，使用分别识别CD298和β2微球蛋白的两个克隆的组合。将抗体偶联到相同的寡核苷酸上，预混合后按照类似于CITE-seq工作流程的优化方案使用。
β2-微球蛋白（β2M）是一种12 kD非多态性Ig样蛋白。它是一种非膜锚定糖蛋白，与MHC I类分子的39-44 kD多态性重链非共价结合，形成HLA I类抗原复合物。与 HLA I 级相关，β2M 在所有白细胞、血小板、内皮细胞和上皮细胞上表达。β2M在控制MHC I类分子稳定性和促进抗原结合和将抗原呈递给CD8+ T细胞的CD3 / TCR复合物方面起着至关重要的作用。
CD298或β3 Na + / K + ATP酶是一种42 kD II型跨膜蛋白，也称为ATP1B3。Na+/K+ ATP酶是一种完整的质膜蛋白，由一个α和一个β亚基组成。已经报道了α的四种亚型和β亚基的三种亚型。Na + / K + ATP酶通过将Na +和K +离子以相反的方向泵送过细胞质膜，将ATP水解与离子梯度的发展偶联。它具有广泛的组织分布，包括所有白细胞和许多其他组织。
TotalSeq™ anti-human Hashtag reagents are designed to label most human cells, using a combination of two clones that recognize CD298 and β2 microglobulin, respectively. The antibodies are conjugated to the same oligonucleotide, pre-mixed to be used following an optimized protocol similar to the CITE-seq workflow.

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

CD298 or the β3 Na+/K+ ATPase, is a 42 kD type II transmembrane protein, also known as ATP1B3. An integral plasma membrane protein, Na+/K+ ATPase is composed of one α and one β subunits. Four isoforms of the α and three isoforms of the β subunits have been reported. Na+/K+ ATPase couples ATP hydrolysis to the development of an ionic gradient by pumping Na+ and K+ ions in opposite directions across the cell plasma membrane. It has broad tissue distribution, including all leukocytes and many other tissues.

Product Details
Verified Reactivity    Human
Antibody Type   Monoclonal
Host Species   Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.

Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
Concentration   0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application    PG - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.

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