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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
TotalSeq™-C0162 anti-human CD64 Antibody
- CAS号：
TotalSeq™-C0162 anti-human CD64 Antibody
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
10 ug
TotalSeq™-C0162 抗人 CD64 抗体、TotalSeq™-C0162 anti-human CD64 Antibody详细如下：
Description：
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Other Names：FcγRI, FcR I
Isotype：Mouse IgG1, κ
Isotype Control：TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl
Reactivity：Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application：PG - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes：
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
Application References：
1.McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2.Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3.Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4.Holl V, et al. 2004. J. Immunol. 173:6274.
5.Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6.Cho HJ, et al. 2007. Physiol Genomics 149:60.
7.van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8.Bruhns P, et al. 2008. Blood 113:3716. PubMed
9.Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10.Carter DL, et al. 1999. Cytometry 37:41. (FC)
11.Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12.Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Structure：Ig superfamily, type I glycoprotein, 72 kD
Distribution：Monocytes, macrophages, dendritic cells, activated granulocytes
Function：Phagocytosis, ADCC
Ligand/Receptor：IgG receptor
Cell Type：Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area：Immunology, Innate Immunity
Molecular Family：CD Molecules, Fc Receptors
Antigen References：
1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Description：
CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
Other Names：FcγRI, FcR I
Isotype：Mouse IgG1, κ
Isotype Control：TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl
Reactivity：Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation：The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application：PG - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes：
Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
Application References：
1.McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2.Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
3.Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4.Holl V, et al. 2004. J. Immunol. 173:6274.
5.Hober D, et al. 2002. J. Gen. Virol. 83:2169.
6.Cho HJ, et al. 2007. Physiol Genomics 149:60.
7.van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
8.Bruhns P, et al. 2008. Blood 113:3716. PubMed
9.Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
10.Carter DL, et al. 1999. Cytometry 37:41. (FC)
11.Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
12.Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Structure：Ig superfamily, type I glycoprotein, 72 kD
Distribution：Monocytes, macrophages, dendritic cells, activated granulocytes
Function：Phagocytosis, ADCC
Ligand/Receptor：IgG receptor
Cell Type：Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area：Immunology, Innate Immunity
Molecular Family：CD Molecules, Fc Receptors
Antigen References：
1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
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文献和实验相关实验
CD64 分子 CD64 常用单克隆抗体或代号： MAb32.2,MAb22;(Fcg RI) 主要表达细胞： M [M] 分子质量(kDa)和结构： gp75(IgSF) 功 能： 吞噬、ADCC,Mac活化 CD64 Aka Fc gamma RI High affinity receptor binds to Fc region of IgG
（一）材料： 1．动物：家兔 2．人血清 3．羊毛脂、石蜡油、卡介苗 4．其它：无菌生理盐水，无菌研钵等 （二）方法： 1．弗氏 佐剂 的制备 将羊毛脂与石蜡油按1：5比例混合，高压灭菌。 2．人血清——弗氏完全的制备 将灭菌 佐剂 一份置 研钵 中，边研磨边滴加等量的含卡介苗3-4mg/mL的抗原
抗人肺腺癌单链抗体的构建 (1)将表达载体pFUW80质粒经XbaI和EcoRI双酶切，同样双酶切在pUC19中克隆的VL基因，分别回收后，连接并转化大肠杆菌JM109株，鉴定阳性重组子(可通过XbaI、EcoRI双酶切切下合适大小的带，即VL片段)。 (2)将上述经过鉴定的重组质粒用XhoI、SpeI双酶切，回收后，与同样双酶切的在pUC19中克隆的VH基因相连，再次转化JM109感受态细菌。重组质粒筛选、鉴定同上所述。至此抗人
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TotalSeq™-C0162 抗人 CD64 抗体
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