- 询价
- BioLegend
- 319101
- BioLegend
- 2025年10月28日
5 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
Anti-HU CD3 FITC/(CD16+CD56) PE Cktl
- 供应商：
北京云肽生物科技有限公司
BioLegend 319101 Anti-HU CD3 FITC/(CD16+CD56) PE Cktl
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 319101 Anti-HU CD3 FITC/(CD16+CD56) PE Cktl
产品简介：
产品品牌：BioLegend
产品货号：319101
产品名称：BioLegend 319101 Anti-HU CD3 FITC/(CD16+CD56) PE Cktl
产品规格：50 tests
产品说明：
Clone UCHT1; 3G8; MEM-188;
Regulatory Status RUO
Isotype Mouse IgG1/Mouse IgG2a
描述
CD3：UCHT1抗体与CD3ε的组合表位反应，CD3ε是CD3 / T细胞受体（TCR）复合物的20 kD链，存在于所有成熟的T淋巴细胞，NK-T细胞和一些胸腺细胞上。
CD16：3G8抗体与CD16反应，CD16是一种50-65 kD跨膜或48 kD GPI锚定糖蛋白，是免疫球蛋白超家族的成员。CD16在NK细胞，活化的单核细胞，巨噬细胞，肥大细胞和中性粒细胞上表达。
CD56：MEM-188抗体与CD56的175 - 185 kD亚型N-CAM（神经细胞粘附分子）反应，其在NK细胞和CD3 + T细胞亚群上表达。CD56也在大脑（小脑和皮层）和神经肌肉接头中表达。某些大颗粒淋巴细胞 （LGL） 白血病、小细胞肺癌、神经元源性肿瘤、骨髓瘤和骨髓性白血病也表达 CD56。
CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD16: 3G8 antibody reacts with CD16, a 50-65 kD transmembrane or a 48 kD GPI-anchored glycoprotein that is a member of the immunoglobulin superfamily. CD16 is expressed on NK cells, activated monocytes, macrophages, mast cells and neutrophils.
CD56: MEM-188 antibody reacts with the 175 - 185 kD isoform of CD56, N-CAM (Neural Cell Adhesion Molecule), which is expressed on NK cells and a subset of CD3+ T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56.
Product Details
Isotype Control
Mouse IgG1 FITC/IgG2a PE Isotype Ctrl Cocktail
Verified Reactivity Human
Host Species Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of FITC conjugated UCHT1 and PE conjugated 3G8 and MEM-188 in optimal concentration for two-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
Note:
Single color controls are required to perform instrument compensation and are not included in the kit.
Antigen Details
Distribution
T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes
Cell Type
Mesenchymal Stem Cells, NKT cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family CD Molecules
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 155805 TotalSeq™-A0303 anti-mouse Hashtag 3 M1/42; 30-F11 10 ug
BioLegend 155807 TotalSeq™-A0304 anti-mouse Hashtag 4 M1/42; 30-F11 10 ug
BioLegend 155831 TotalSeq™-B0301 anti-mouse Hashtag 1 M1/42; 30-F11 10 ug
BioLegend 155833 TotalSeq™-B0302 anti-mouse Hashtag 2 M1/42; 30-F11 10 ug
BioLegend 155835 TotalSeq™-B0303 anti-mouse Hashtag 3 M1/42; 30-F11 10 ug
BioLegend 155837 TotalSeq™-B0304 anti-mouse Hashtag 4 M1/42; 30-F11 10 ug
BioLegend 155839 TotalSeq™-B0305 anti-mouse Hashtag 5 M1/42; 30-F11 10 ug
BioLegend 155861 TotalSeq™-C0301 anti-mouse Hashtag 1 M1/42; 30-F11 10 ug
BioLegend 155863 TotalSeq™-C0302 anti-mouse Hashtag 2 M1/42; 30-F11 10 ug
BioLegend 155865 TotalSeq™-C0303 anti-mouse Hashtag 3 M1/42; 30-F11 10 ug
BioLegend 155867 TotalSeq™-C0304 anti-mouse Hashtag 4 M1/42; 30-F11 10 ug
BioLegend 155869 TotalSeq™-C0305 anti-mouse Hashtag 5 M1/42; 30-F11 10 ug
BioLegend 155875 TotalSeq™-C0308 anti-mouse Hashtag 8 M1/42; 30-F11 10 ug
BioLegend 155879 TotalSeq™-C0310 anti-mouse Hashtag 10 M1/42; 30-F11 10 ug
BioLegend 155905 APC anti-mouse CD183 (CXCR3) S18001A 25 ug
BioLegend 156104 PE anti-mouse TIGIT (Vstm3) 4D4/mTIGIT 100 ug
BioLegend 156304 PE anti-mouse TCR vβ8.3 1B3.3 100 ug
BioLegend 156402 Purified anti-mouse CD32 (Fcgr2) S17012B 500 ug
BioLegend 156405 APC anti-mouse CD32 (Fcgr2) S17012B 25 ug
BioLegend 156406 APC anti-mouse CD32 (Fcgr2) S17012B 100 ug
BioLegend 156407 FITC anti-mouse CD32 (Fcgr2) S17012B 25 ug
BioLegend 156503 PE anti-mouse NK-1.1 S17016D 25 ug
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750（每次测试 5μL），以及 CD16-FITC（每次测试 20 μL），并在冰上孵育 30 分钟。10. 在 4℃ 条件下，300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集：1. 创建新补偿值。2. 如适用
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
抗体的一种形式。这类抗体片段通常是由一个启动子控制下的两个顺反子表达产物间反向配对而成具有两个抗原结合位点的双特异抗体片段。它的基本原理是同一顺反子中VH和VL因Linker太短（通常只有5-12个氨基酸），产生空间位阻，不能配对，只能与另一顺反子上的VH和VL配对，从而形成二聚体。通常使用的效应细胞主要有T细胞（CD2，CD3）、NK细胞（CD16）、单核/巨噬细胞（CD64）、树突状细胞（CD64）和激活的髓系细胞（CD64，CD89），由于存在以下三方面的原因，使得以T细胞为效应细胞要比其它两
技术资料暂无技术资料 索取技术资料
BioLegend 319101 Anti-HU CD3 FITC/(CD16+CD56) PE Cktl
询价
