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- 库存：
99
- 英文名：
Biotin anti-mouse/rat CD81
- 供应商：
北京云肽生物科技有限公司
BioLegend 104903 Biotin anti-mouse/rat CD81
北京云肽生物科技有限公司作为BioLegend的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国BioLegend公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长，但它聚集了一批来自PharMingen公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 104903 Biotin anti-mouse/rat CD81
产品简介：
产品品牌：BioLegend
产品货号：104903
产品名称：BioLegend 104903 Biotin anti-mouse/rat CD81
产品规格：50 µg
产品说明：
Clone Eat-2 (See other available formats)
Regulatory Status RUO
Other Names TAPA-1
Isotype Armenian Hamster IgG1, κ
描述
CD81是tetraspanin超家族（TM4SF）的26 kD非糖基化成员，也称为TAPA-1。CD81 在 T 细胞和 B 细胞、NK 细胞、树突状细胞、胸腺细胞、内皮细胞和成纤维细胞上表达。CD81通过VLA-4整合素诱导B细胞粘附，并已被证明在早期T细胞发育中发挥作用。CD81 与多分子复合物中的其他几种细胞表面蛋白结合，包括 B 细胞中的 CD19、CD21、CD20、CD37、CD53 和 CD82，以及 T 细胞中的 CD4、CD8 和 CD82。
CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.
Product Details
Isotype Control Biotin Armenian Hamster IgG Isotype Ctrl
Verified Reactivity Mouse, Rat
Antibody Type Monoclonal
Host Species Armenian Hamster
Immunogen CD81+ mouse B lymphoma 38C13
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2.
Antigen Details
Structure TM4SF tetraspanin, 26 kD
Distribution T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts
Function Regulates cell growth
Cell Type B cells, Dendritic cells, Endothelial cells, Fibroblasts, NK cells, T cells, Thymocytes
Biology Area Immunology
Molecular Family Adhesion Molecules, CD Molecules
以下是BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
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BioLegend 100542 Brilliant Violet 570™ anti-mouse CD4 RM4-5 50 ug
BioLegend 100543 Brilliant Violet 421™ anti-mouse CD4 RM4-5 125 uL
BioLegend 100544 Brilliant Violet 421™ anti-mouse CD4 RM4-5 500 uL
BioLegend 100545 Brilliant Violet 650™ anti-mouse CD4 RM4-5 125 uL
BioLegend 100546 Brilliant Violet 650™ anti-mouse CD4 RM4-5 500 uL
BioLegend 100550 Brilliant Violet 711™ anti-mouse CD4 RM4-5 500 uL
BioLegend 100551 Brilliant Violet 785™ anti-mouse CD4 RM4-5 125 uL
BioLegend 100552 Brilliant Violet 785™ anti-mouse CD4 RM4-5 50 ug
BioLegend 100555 Brilliant Violet 650™ anti-mouse CD4 RM4-5 50 ug
BioLegend 100559 Brilliant Violet 510™ anti-mouse CD4 RM4-5 50 ug
BioLegend 100561 Purified anti-mouse CD4 (Maxpar® Ready) RM4-5 100 ug
BioLegend 100566 PE/Dazzle™ 594 anti-mouse CD4 RM4-5 100 ug
BioLegend 100567 APC/Fire™ 750 anti-mouse CD4 RM4-5 25 ug
BioLegend 100569 TotalSeq™-A0001 anti-mouse CD4 RM4-5 10 ug
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文献和实验相关实验
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
3 mAb,PE-Cy5 50T/100T 1200/1800 L0005 Mouse anti-Human CD3 mAb,APC 50T/100T 1000/1500 L0006 Mouse anti-Human CD3 mAb,Biotin 50T/100T
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BioLegend 104903 Biotin anti-mouse/rat CD81
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