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- 英文名:
BioLegend 315804 FITC anti-human CD177
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国 BioLegend 公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长,但它聚集了一批来自 PharMingen 公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
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BioLegend 315804 FITC anti-human CD177
产品简介:
产品品牌:BioLegend
产品货号:315804
产品名称:BioLegend 315804 FITC anti-human CD177
产品规格:100 tests
产品说明:
Clone MEM-166 (See other available formats)
Regulatory Status RUO
Workshop HCDM listed
Other Names
Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype Mouse IgG1, κ
描述
CD177 也称为中性粒细胞特异性抗原 1、NB1 和真性红细胞增多症 1。它是uPAR家族的成员,是一种分子量为60kD的GPI连接的细胞表面糖蛋白。CD177在粒细胞和骨髓祖细胞(早期成红细胞,巨核细胞)上表达。它被认为参与对中性粒细胞的同种异体和自身免疫反应。
CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.
Product Details
Isotype Control FITC Mouse IgG1, κ Isotype Ctrl
Verified Reactivity Human, Cynomolgus, Rhesus
Antibody Type Monoclonal
Host Species Mouse
Immunogen Human granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.
Antigen Details
Structure uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution
Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)
Function
Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification Glycosylated
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area Immunology
Molecular Family CD Molecules
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文献和实验Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
cells in human pluripotent stem cell cultures, whereas hCD29 recognizes an antigen expressed on undifferentiated and many differentiated cells. A distinguishing feature of hCD29 in live?cell staining is that its antigen is precluded from detection
抗体的名字,注意中英文名字、它名、亚型等信息。 (2)确定你的实验类型,Elisa,WB,IHC,ICC,还是FACS。一般抗体的说明书都会列出该抗体经验证过适用于何种实验的类型,如果抗体说明 书没有提及的应用类型,并不意味着该抗体不适用于此种分析应用类型,而仅是说明尚未经过此种实验验证,请根据说明书列出的已验证的类型来选择适合你实验的 抗体。 (3)确定实验样本的种属,Human,Mouse,还是Rat。一般抗体说明书都列出该抗体经试验验证过适用于何种物种
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