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- 2025年10月21日
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- 库存：
99
- 英文名：
FITC anti-mouse H-2Kb/H-2Db
- 供应商：
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为BioLegend的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国BioLegend公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend虽然成立的时间不长，但它聚集了一批来自PharMingen公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。BioLegend公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 114606 FITC anti-mouse H-2Kb/H-2Db
产品简介：
产品品牌：BioLegend
产品货号：114606
产品名称：BioLegend 114606 FITC anti-mouse H-2Kb/H-2Db
产品规格：500 µg
产品说明：
Clone 28-8-6 (See other available formats)
Regulatory Status RUO
Other Names MHC class I
Isotype Mouse (C3H) IgG2a, κ
描述
28-8-6抗体与H-2Kb/H-2Db单倍型小鼠的有核细胞上表达的H-2Kb和H-2Db MHC I类同种抗原反应。H-2Kb/H-2Db参与向表达CD3/TCR和CD8蛋白的T细胞的抗原呈递。28-8-6抗体与H-2Dd MHC I类同种抗原交叉反应，但不与f、k、p、q、r、s单倍型的同种抗原反应。
The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
Product Details
Isotype Control FITC Mouse IgG2a, κ Isotype Ctrl
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Mouse
Immunogen C3H.SW mouse splenocytes
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm)
Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of *-fixed frozen sections.
Antigen Details
Structure MHC class I
Distribution Nucleated cells of the H-2Kb/H-2Db haplotype
Function Antigen presentation
Ligand/Receptor CD3/TCR, CD8
Biology Area Immunology, Innate Immunity
Molecular Family MHC Antigens
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文献和实验A general protocol for staining cell for cytometry analysis
. No. 65875X, 65875H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 4. 1X PBS Buffer: 8 g NaCl; 0.2 g KCl; 1.44 g Na 2 HPO 4 • 7H 2 0; 0.24 g KH 2 PO 4 ; H 2 0 to 1 liter. Adjust pH to 7.2, autoclave and store at RT.
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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