产品名称
PLBL2 / PLBD2 ELISA Kit, Chinese hamster
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Cell supernatant
灵敏度
60 pg/mL
线性范围
390.63-25000 pg/mL
特异性
Recognizes both recombinant Chinese Hamster (CHO) PLBL2/PLBD2
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This PLBL2 / PLBD2 ELISA Kit, Chinese hamster is an enzyme-linked immunosorbent assay for the quantitative measurement of Chinese hamster PLBL2 / PLBD2 protein in Cell supernatant . It contains recombinant Chinese hamster PLBL2 / PLBD2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
PLBL2 / PLBD2 ELISA Kit, Chinese hamster: 图片
PLBL2 / PLBD2 背景信息
PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.
全称
phospholipase B domain containing 2
参考文献
Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.