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- 详细信息
- 文献和实验
- 技术资料
- 库存:
88
- 供应商:
武汉天德生物科技有限公司
- 检测范围:
1.56-100 ng/mL
- 检测方法:
ELISA
- 应用:
用于血清、血浆或其他相关生物液体中花生四烯酸的测定。
- 适应物种:
详询
- 标记物:
Biotin
- 样本:
血清、血浆及相关液体
- 规格:
96TESTS
TD751021
花生四烯酸(AA)ELISA试剂盒
| 英文名 | AA(Arachidonic Acid) ELISA Kit | ||
| 别名 | ARA | ||
| 相关类别 | ELISA | 储存 | 冷藏(2-8℃) |
应用
用于血清、血浆或其他相关生物液体中花生四烯酸的测定。
For the determination of Universal AA(Arachidonic Acid) concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是竞争法酶联免疫吸附检测技术(ELISA)。测定样品中花生四烯酸水平。向预先包被了花生四烯酸抗原的酶标孔中,加入标准品和样本,温育后,加入生物素标记的抗花生四烯酸抗体。再与HRP标记的链霉亲和素结合,形成免疫复合物,再经过温育和洗涤,去除未结合的酶,然后加入显色底物TMB,产生蓝色,并在酸的作用下转化成最终的黄色。最后,在450 nm处测定反应孔样品吸光度(OD)值,样本中的花生四烯酸浓度与OD值成反比,通过绘制标准曲线计算出样本中花生四烯酸的浓度。 |
| Purpose | This assay employs the Competitive enzyme immunoassay technique. Determine the level of Universal AA(Arachidonic Acid) in the sample. Add standard and sample to the microplate that have been pre-coated with Universal AA(Arachidonic Acid) antigen. After incubation, add Biotin-conjugated anti-Universal AA(Arachidonic Acid) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Universal AA(Arachidonic Acid) in the sample was proportional to the OD value. The concentration of Universal AA(Arachidonic Acid) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 0.94 ng/mL |
| Sensitivity | 0.94 ng/mL |
| 检测范围 | 1.56-100 ng/mL |
| Examination range | 1.56-100 ng/mL |
| 特异性 | 可检测样本中的花生四烯酸,且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Universal AA(Arachidonic Acid) in samples. No significant cross-reactivity or interference between Universal AA(Arachidonic Acid) and analogues was ob/served. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次,以及在不同的板子上分别检测20次,板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人花生四烯酸 (AA)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 AA 单抗包被于酶标板上,标准品和样品中的 AA 与单抗结合,加入生物素化的抗人 AA ,形成免疫复合物连接在板上,辣根
Neutralizing Arachidonic Acid 中和花生四烯酸
Contributor: Suprya JayadevDate: August 21, 1991NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.--> The solution should appear yellow since the free
仍然是一个非常好的选择。 美誉度 一个品牌不光要有知名度,更重要的是美誉度。通过行业调查公司以及网络,相关宣传资料等可以找到美誉度高的 ELISA 试剂盒供应商,这个反映的不仅是产品质量的问题,对供应商的售前售后服务也是一个非常严格的检验。目前国际上主要的 ELISA 供应商有 R&D 细胞因子方面的试剂盒,Bender 粘附分子方面的试剂盒,Cayman 花生四烯酸类产品的试剂盒,Merck(Linco) 内分泌研究方面的试剂盒,Invitrogen(Biosource
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