美天旎Miltenyi130-104-186 FeraTrack直接MRI造影剂,产品简介: 产品品牌:美天旎Miltenyi 产品货号:130-104-186 产品名称:FeraTrack直接MRI造影剂 产品规格: for 25×106 total cells
美天旎Miltenyi130-104-186 FeraTrack直接MRI造影剂,产品说明: Magnetic resonance imaging (MRI) is the most frequently used technique for serial in vivo cell tracking applications due to high resolution of soft tissues, which makes it especially useful for imaging of the brain, muscles, or the heart.¹ In order to improve detectability of transplanted cells and produce a strong contrast against surrounding tissue, intracellular labeling of cells with iron oxide particles before transplantation has been described.² FeraTrack Direct Contrast Particles are SPIO nanoparticles specifically formulated for direct uptake from cell culture medium without the need of transfection reagent. Uptake of FeraTrack Direct Contrast Particles was proven by Prussian Blue staining, MRI, and electron microscopy. Biocompatibility was assured by cell expansion and differentiation assays of intracellularly labeled cells. Once within cells, SPIOs can induce decreased signal intensity on T1, T2, and T2*-weighted images.³ Applications FeraTrack Direct Contrast Particles are optimized for in vitro labeling of cell lines (e.g. NIH-3T3, Jurkat), primary cells (e.g. T cells), and stem cells (e.g. mesenchymal stem cells) independent of an additional transfection reagent. 磁共振成像(MRI)是体内细胞连续跟踪最常用的技术应用,由于软组织的高分辨率,这使得它特别有用对于大脑,肌肉,或心脏的成像。¹为了提高移植细胞的可探测性,并产生与周围组织的强烈对比,细胞内标记与氧化铁颗粒在移植前已经被描述。²FeraTrack直接造影粒子是SPIO纳米粒子,专门为直接从细胞培养基中摄取而配制,而不需要转染试剂。Prussian蓝染色、MRI和电子显微镜证实了FeraTrack Direct Contrast Particles的摄取。 通过细胞内标记细胞的扩增和分化试验来保证生物相容性。 一旦在细胞内,SPIOs可以诱导T1、T2和T2*加权图像上的信号强度降低。³ 应用 FeraTrack Direct Contrast Particles被优化用于体外标记细胞系(例如。 NIH-3T3,Jurkat),原代细胞(例如, 和干细胞(例如, 间充质干细胞)而独立于额外的转染试剂。
组成成分: 1×200 µL FeraTrack™ Direct Contrast Particles MRI cell tracking agent (superparamagnetic iron oxide [SPIO] nanoparticles) or 5×200 µL FeraTrack™ Direct Contrast Particles MRI cell tracking agent (superparamagnetic iron oxide [SPIO] nanoparticles)