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- 翌圣生物（Yeasen）
- 99105ES60
- 上海
- 2025年11月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
有效期1年
- 保存条件：
+15°C to +30°C
- 英文名：
Bis-tris propane
- 供应商：
翌圣生物科技(上海)股份有限公司
- CAS号：
64431-96-5
- 库存：
大量
- 规格：
25g
Bis-tris propane
Product Description（产品描述）
Bis-Tris propane is a zwitterionic buffer that is used in biochemistry and molecular biology. It has an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value.
A review of DNA polymerase fidelity and its role in the polymerase chain reaction (PCR) discusses the use of Bis-Tris propane as a suitable buffer. The use of a Bis-Tris propane/HCl buffer for the stabilization of farnesyl diphosphate isolated from a mutant strain of Saccharomyces cerevisiae has been described.
BisTris propane buffer has been used in a study of the effects of buffer identity on electric signals of lightexcited bacteriorhodopsin. An investigation of the MgATPase activity of the myosin subfragment monomer in Bis-Tris propane buffer has been reported. The effect of buffer identity on the kinetics of the restriction enzyme EcoR V has been studied in various buffers, including Bis-Tris propane.
Bis-Tris propane has been used in protein crystallization, such as in the crystallization of glutamate dehydrogenase from Thermotoga maritima and of pullulanase type I from Fervidobacteriumpennivorans Ven The analysis of Langmuir and Langmuir-Blodgett monolayers of organophosphorus acid anhydrolase in different subphases, including Bis-Tris propane buffer, has been reported.The preparation and phosphodiester hydrolysis activity of complexes of lanthanum and Bis-Tris propane have been investigated.
分子式：C11H26N2O6
分子量：282.3
CAS Number: 64431-96-5
pKa1: 6.8 (25 °C)
pKa2: 9.0 (25 °C)
∆pK/∆T = -0.03
别名：1,3-bis[tris(hydroxymethyl) methylamino]propane
Precautions and Disclaimer（注意事项及免责声明）
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions（准备说明）
This product is soluble in water (428 mg/ml), yielding a clear, colorless solution.
Storage/Stability （储存/稳定性）
Solutions of this product are expected to be stable to autoclaving. Solutions stored at 2-8 °C are stable for
months.
Ordering Information（订购信息）
| 货号 |
名称 |
产地 |
CAS 号码 |
规格 |
报价 |
| 99105ES60 |
Bis-tris propane |
YEASEN |
64431-96-5 |
100G |
609 |
| 99105ES60 |
Bis-Tris Propane |
YEASEN |
64431-96-5 |
25g |
480 |
其他相关产品：每次可供应100kg的量
| 货号 |
名称 |
产地 |
CAS 号码 |
规格 |
报价 |
| 99104ES25 |
Bis-Tris |
YEASEN |
6976-37-0 |
25g |
328 |
| 99104ES25 |
Bis-Tris |
YEASEN |
6976-37-0 |
250g |
2080 |
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文献和实验使用 3～5 次。 转移缓冲液 1X 电转液： 加蒸馏水溶解定容至 1000 mL 溶解后 4 ℃ 保存，1 次溶液可重复使用 3～5 次。 TBS 缓冲液 加蒸馏水溶解，冰醋酸调 pH 至 7.2，定容至 1000 mL。室温保存。 洗脱液 TBST 溶液 TBS 溶液 1000 mL ，1 mL 吐温 20，混匀后即可使用，最好现用现配。 封闭液（含 5% 脱脂奶粉的 TBST 缓冲液） 溶解后使用，一次性使用。 RIPA（中）裂解液 Tris-base、NaCl、EDTA、SDS
各种pH值的Tris缓冲液的配制 各种pH值的Tris缓冲液的配制 所需pH值（25
一、TBS的配制 0.05 M TBS ( pH7.4 ) 的配制： Tris ( 三羟甲基胺基甲烷 ) 12.1g NaCl 17.5g 加蒸馏水 1500ml 磁性搅拌下滴加浓HCl至pH为7.4，再加蒸馏水至2000ml，即可。 如需含1% Triton X-100，则在滴加HCl前先加入20
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