Mouse IgG1 (N297A) kappa, Isot

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Retroviral Gene Expression Control in Primary Organoid Cultures

of transgenic mouse lines. Establishing such animal models is laborious, expensive, and time?consuming. Ever?expanding endodermal organoids, grown in an in vitro 3?D epithelial culture system, faithfully recapitulate the in vivo counterpart and represent