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- 技术资料
- 供应商:
联科生物
- 库存:
99
- 样本:
血清,血浆,细胞培养上清及其他生物学样本
- 标记物:
HRP
- 适应物种:
Rat
- 应用:
ELISA
- 检测方法:
双抗夹心法
- 检测范围:
46.88 pg/mL - 3000 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1600.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2650.0 |
描述
| 商品名 | Rat C-Reactive Protein/CRP ELISA Kit (大鼠C-反应蛋白 ELISA试剂盒) |
|---|---|
| 检测方法 | 双抗夹心法 |
| 精密度 | 板内变异系数:4.6% - 5.0%;板间变异系数:5.7% - 6.6% |
| 样本类型 | 血清,血浆,细胞培养上清及其他生物学样本 |
| 检测样本体积 | 100 μL |
| 灵敏度 | 3.13 pg/mL |
| 检测范围 | 46.88 pg/mL - 3000 pg/mL |
| 回收率 | 80% - 121% |
| 平均回收率 | 1.08 |
| 板式 | 96孔板,可拆 |
| 保存 | 试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠CRP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CRP与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CRP的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
标准曲线
分子信息
概述大鼠 CRP, C-reactive protein, PTX1 靶点信息CRP 分子靶点信息概述
- 分子名:CRP, C-reactive protein
- 基因家族:Short pentraxins
- 别名:PTX1
- 全称:pentraxin 1; C-reactive protein, pentraxin-related
CRP 分子靶点综述
C-反应蛋白(CRP)是在血浆中发现的一种环状、五聚体蛋白。肝脏对巨噬细胞和脂肪细胞释放的因子产生应答后可合成CRP。CRP是一种肝源性的急性蛋白,在巨噬细胞和T细胞分泌白介素6后增加,其生理作用是与死细胞或濒死细胞(和某些类型的细菌)表面的溶血磷脂胆碱结合,以通过C1Q复合物来激活补体系统。它与C-肽(胰岛素)或蛋白C(血凝)不相关。CRP通常作为炎症标志物,当机体受到炎症感染时,CRP水平会上升。最近的研究表明,患者具有较高基础水平的CRP,通常伴随着一个较高糖尿病、高血压和心血管疾病的发生率。
大鼠 Rat Crp 分子靶点信息
- 分子名:Crp, C-reactive protein
- 别称:
- Aa1249
- Ab1-341
- Ab2-196
- Ac1-114
- Ac1262
- Ac2-069
- Ba2-693
- C-reactive protein member of the pentraxin family
- C-reactive protein, member of the pentraxin family
- C-reactive protein, pentraxin-related
- C-reactive protein, petaxin related
- 基因序列:NCBI_Gene: 25419
- 蛋白序列:
- UniprotKB: A0A0G2K8V5
- UniprotKB: H6X2V2
- UniprotKB: H6X2V3
- UniprotKB: H6X2V4
- UniprotKB: H6X2V7
- UniprotKB: H6X2V8
- UniprotKB: H6X2V9
- UniprotKB: H6X2W0
- UniprotKB: H6X2W1
- UniprotKB: H6X2W2
- UniprotKB: H6X2W3
- UniprotKB: H6X2W4
- UniprotKB: H6X2W5
- UniprotKB: H6X2W6
- UniprotKB: H6X2W7
- UniprotKB: H6X2X0
- UniprotKB: P48199
- UniprotKB: Q63133
- UniprotKB: Q63134
大鼠 Rat Crp 靶点分子功能(预测)
Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).
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大鼠胱抑素C(Cystatin C)ELISA试剂盒 说明书
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