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- 询价记录
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Klenow Fragment (3'→5' exo-)
- 保质期:
2年
- 供应商:
翌圣生物科技(上海)股份有限公司
- 保存条件:
-25~-15℃保存
- 规格:
200 U(14460ES70)/1000 U(14460ES80)/5000 U(14460ES90)
| 规格: | 200 U(14460ES70) | 产品价格: | ¥468 |
|---|---|---|---|
| 规格: | 1000 U(14460ES80) | 产品价格: | ¥1898 |
| 规格: | 5000 U(14460ES90) | 产品价格: | ¥7568 |
产品描述:
Klenow (3'→5'exo-)DNA聚合酶是DNA聚合酶I的E. coli DNA聚合酶I的蛋白水解产物,保留了DNA聚合酶 I的DNA聚合酶活性,但失去了5'→3'核酸外切酶活性,该酶通过突变进一步去除了3'→5'核酸外切酶活性。可以用于随机引物法进行DNA标记、双脱氧法DNA序列测定等。
产品特色:
- 5'→3'DNA聚合酶活性;
- 缺失5'→3'核酸外切酶活性;
- 缺失3'→5'核酸外切酶活性。
应用案例:
- E.coli基因组DNA残留< <1 copies/50 U
图1. E.coli 基因组DNA残留检测
- 应用于NGS建库,产量高于进口品牌
图2.产品建库产量结果比较
- 产品更稳定:37°C放置14 d剩余酶活显著高于进口品牌
图3. 产品加速稳定性结果
储存条件:
-25~-15℃保存,有效期2年。
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询价记录
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文献和实验Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity
Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
Historically, DNA sequencing by primed synthesis and chain terminators has been performed using the Klenow fragment of DNA polymerase 1(1 . This enzyme was derived from E. coli . DNA Pol I which, in addition to polymerase activity, has both 3′–
Mainly as a result of the booming interest in apoptosis and of the discovery of the occurrence of massive DNA breaks as a major feature of this type of cell death, during the past few years polymerase-based assays for detection of DNA breaks












