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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Microcin H47 (mchB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100ug/20ug
| 规格: | 100ug | 产品价格: | ¥16200.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥9720.0 |
Research Areas
CancerUniprot ID
P62530Gene Names
mchBAlternative Name(s)
(MccH47)Lead Time
3-7 business daysOrganism
Escherichia coliSource
in vitro E.coli expression systemExpression Region
16-75aaProtein Length
Full Length of Mature ProteinTag Info
N-terminal 10xHis-taggedTarget Protein Sequence
GGAPATSANAAGAAAIVGALAGIPGGPLGVVVGAVSAGLTTAIGSTVGSGSASSSAGGGSMW
10.9 kDaPurity
Greater than 85% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
Bactericidal antibiotic. Active on bacteria phylogenetically related to the producing strain.Function
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文献和实验Resolving Bottlenecks for Recombinant Protein Expression in E. coli
Escherichia coli is widely used as an expression system for production of recombinant proteins of prokaryotic and eukaryotic origin. A large body of knowledge has accumulated throughout the last few decades
Screening for the Expression of Soluble Recombinant Protein in Escherichia coli
Protein expression and purification have traditionally been time-consuming, case-specific endeavors, and are considered to be the greatest bottlenecks in most proteomics pipelines. Escherichia coli (E. coli ) is the most convenient and cost
Automated Recombinant Protein Expression Screening in Escherichia coli
, Strasbourg for performing recombinant protein expression screening in Escherichia coli . The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.
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