mutS/mutS蛋白Recombinant Aquifex

纯度：

Greater than 85% as determined by SDS-PAGE.

基因名：

mutS

Uniprot No.：

O66652

别名：

/

种属：

Aquifex aeolicus (strain VF5)

蛋白长度：

Full Length

来源：

E.coli

分子量：

146.1 kDa

表达区域：

1-859aa

氨基酸序列：

MEKSEKELTPMLSQYHYFKNQYPDCLLLFRLGDFYELFYEDAYIGSKELGLVLTSRPAGKGKERIPMCGVPYHSANSYIAKLVNKGYKVAICEQVEDPSKAKGIVKREVVRVITPGTFFERDTGGLASLYKKGNHYYVGYLNLAVGEFLGAKVKIEELLDLLSKLNIKEILVKKGEKLPEELEKVLKVYVSELEEEFFEEGSEEILKDFGVLSLQAFGFEEDTYSLPLGAVYKYAKTTQKGYTPLIPRPKPYRDEGFVRLDIKAIKGLEILESLEGRKDISLFKVIDRTLTGMGRRRLKFRLLSPFRSREKIERIQEGVQELKENREALLKIRQILEGMADLERLVSKISSNMATPRELVYLKNSLKKVEELRLLLLELKAPIFKEILQNFEDTKKIINDIEKTLVEDPPLHVKEGGLIREGVNAYLDELRFIRDNAETYLREYEKKLRQETGIQSLKIGYNKVMGYYIEVTKPNLKYVPSYFRRRQTLSNSERFTTEELQRLEEKILSAQTRINDLEYELYKELRERVVKELDKVGNNASAVAEVDFIQSLAQIAYEKDWAKPQIHEGYELIIEEGRHPVIEEFVENYVPNDTKLDRDSFIHVITGPNMAGKSSYIRQVGVLTLLSHIGSFIPARRAKIPVVDALFTRIGSGDVLALGVSTFMNEMLEVSNILNNATEKSLVILDEVGRGTSTYDGIAISKAIVKYISEKLKAKTLLATHFLEITELEGKIEGVKNYHMEVEKTPEGIRFLYILKEGKAEGSFGIEVAKLAGLPEEVVEEARKILRELEEKENKKEDIVPLLEETFKKSEEAQRLEEYEEIIKKIEEIDIGNTTPLQALLILAELKKKCSFSKKESGA

蛋白标签：

N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

13-23 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

/

Product types:

Developed Protein

Biological_Activity:

/

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998)

Function:

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