/MELT重组蛋白MEL（MLT)（Allergen Api

英文名称：

Recombinant Apis mellifera Melittin (MELT)

品名简称：

Recombinant Apis mellifera MELT protein

货号：

CSB-EP355665DNK

规格:

20μg

价格：

3324

LC-MS/Western Blot：

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SDS-PAGE：

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

纯度：

Greater than 90% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

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基因名：

MELT

Uniprot No.：

P01501

别名：

MEL (MLT) (Allergen Api m 3) (Allergen Api m III) (Allergen: Api m 4)

种属：

Apis mellifera (Honeybee)

蛋白长度：

Full Length of Mature Protein

来源：

E.coli

分子量：

2.8 kDa

表达区域：

44-69aa

氨基酸序列：

GIGAVLKVLTTGLPALISWIKRKRQQ

蛋白标签：

Tag-Free

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

13-23 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

Melittin: Main toxin of bee venom with strong hemolytic activity and antimicrobial activity . It has enhancing effects on bee venom phospholipase A2 activity . This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis . It acts as a voltage-gated pore with higher selectivity for anions over cations . The ion conductance has been shown to be voltage-dependent . Self-association of melittin in membranes is promoted by high ionic strength, but not by the presence of negatively charged lipids . In vivo, intradermal injection into healthy human volunteers produce sharp pain sensation and an inflammatory response . It produces pain by activating primary nociceptor cells directly and indirectly due to its ability to activate plasma membrane phospholipase A2 and its pore-forming activity .

功能2：

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文献：

"Identification of a novel melittin isoform from Africanized Apis mellifera venom." Sciani J.M., Marques-Porto R., Lourenco A. Jr., Orsi R.D., Junior R.S., Barraviera B., Pimenta D.C. Peptides 31:1473-1479(2010)

研究领域：

Cell Biology

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