大肠杆菌mcjA重组蛋白(MccJ25)(Class II

英文名称：

Recombinant Escherichia coli Microcin J25 (mcjA)

品名简称：

Recombinant E.coli mcjA protein

货号：

CSB-EP894911ENL

规格:

20μg

价格：

2328

LC-MS/Western Blot：

/

SDS-PAGE：

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

纯度：

Greater than 90% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

/

基因名：

mcjA

Uniprot No.：

Q9X2V7

别名：

(MccJ25)(Class II lasso peptide)(Ribosomally synthesized and post-translationally modified peptide)(RiPP)

种属：

Escherichia coli

蛋白长度：

Full Length of Mature Protein

来源：

E.coli

分子量：

17.5 kDa

表达区域：

38-58aa

氨基酸序列：

GGAGHVPEYFVGIGTPISFYG

蛋白标签：

N-terminal 6xHis-KSI-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). Inhibits transcription by binding deep within RNAP secondary channel, where it sterically blocks the folding of the trigger loop, which is essential for efficient catalysis. In addition, it also seems to restrict access of nucleotide substrates to the catalytic center, and shows a partially competitive mode of inhibition with them. Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains. Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption. Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins.

功能2：

/

文献：

"The antibacterial action of microcin J25: evidence for disruption of cytoplasmic membrane energization in Salmonella newport." Rintoul M.R., de Arcuri B.F., Salomon R.A., Farias R.N., Morero R.D. FEMS Microbiol. Lett. 204:265-270(2001)

研究领域：

Others

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