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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Human Oncostatin-M (OSM), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
ImmunologyUniprot ID
P13725Gene Names
OSMAlternative Name(s)
(OSM)Lead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
Mammalian cellExpression Region
26-221aaProtein Length
PartialTag Info
N-terminal hFc-taggedTarget Protein Sequence
AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRMW
44.1 kDaPurity
Greater than 90% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验水平。根据试剂盒说明书,用生长于或未生长于MEFs上的未分化ES细胞制备细胞核提取物。用Bradford检测对蛋白质进行定量分析。STAT3活化水平根据产品说明书而确定,然后根据总蛋白水平进行标准化处理。 小鼠嵌合体的生产:所有程序由密西根大学动物使用和照顾委员会批准。根据国家研究委员会关于实验动物照顾和使用指南概述的原则和程序对动物提供照顾。在含有LIF或者OSM的培养基中连续传代5次后,用胰蛋白酶处理细胞,然后将16个ES细胞显微注射到囊胚中。囊胚取自与C57
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人抑瘤素 ( OSM )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Oncostatin M / OSM 单抗包被于酶标板上,标准品和样品中的 OSM与单抗结合,加入生物素化的抗人
2个HCRF结构域 LIFR/gp130 20~30pM OSM gp130 1个HCSF结构域 500pM gp130/LIFR 5pM CNTF CNTFRα链 1个ICSF结构域 10nM CNTFRα/gp130、LIFR 100~200pM 1个HCSF结构域(GPI锚) 注:(1)LIF:leukaemia inhibitory factor;OSM:oncostatin M;CNTF:ciliary neurotrophic factor. (2)IGSF
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