GLP1R/GLP1R蛋白Recombinant Human

纯度：

Greater than 95% as determined by SDS-PAGE.

基因名：

GLP1R

Uniprot No.：

P43220

别名：

(GLP-1 receptor)(GLP-1-R)(GLP-1R)

种属：

Homo sapiens (Human)

蛋白长度：

Partial

来源：

Mammalian cell

分子量：

19.3 kDa

表达区域：

24-145aa

氨基酸序列：

RPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLY

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels.

内毒素：

Less than 1.0 EU/ug as determined by LAL method.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

Active Protein & In Stock Protein

Biological_Activity:

Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R at 2 μg/mL can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA1HU), the EC50 is 54.54-94.23 ng/mL.

Research Areas:

Cardiovascular

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"Characterization of the 5-HT6 receptor coupled to Ca2+ signaling using an enabling chimeric G-protein." Zhang J.Y., Nawoschik S., Kowal D., Smith D., Spangler T., Ochalski R., Schechter L., Dunlop J. Eur J Pharmacol 472:33-38(2003)

Function:

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