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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dermatophagoides farinae Der f 9 allergen
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dermatophagoides farinae Der f 9 allergen品名简称:
Recombinant Dermatophagoides farinae Der f 9 allergen protein货号:
CSB-EP3962DCO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
A1KXG4别名:
(Fragment)种属:
Dermatophagoides farinae (American house dust mite)蛋白长度:
Full Length来源:
E.coli分子量:
31.4 kDa表达区域:
1-224aa氨基酸序列:
IVGGENASPGDAIYQIALLRKDSFTCGGSLISSRTVLTAAHCVFGDEASPSYFKIRYNTLDRTNGPTIGVSKIYRHSLYSSTTIDYDVATLILSEPFTPSANADIISLTTAEPADNTQLQLTGWGRLKSGGTIPTLLQIATISKMSRTKCSNIWGSVNAITNRMLCAHSKKQSACNGDCGGPLVYNGHLVGVVSWGPSTCLSNTYPTIYSNVATLRNWIISNTV蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"Identification and cloning of group 9 allergen from Dermatophagoides farinae expressed sequence tags." Chew F.T., Ong S.T., Shang H., Bi X., Lim S.H., Tsai L.-C., Lee B.W.研究领域:
Others请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白
层析复性蛋白质 李明1 苏志国1 Janson Jan-Christer2 (1 生化工程国家重点实验室,中国科学院过程工程研究所,北京,100080 2 表面生物技术系,Uppsala 大学,Uppsala, 瑞典) 摘要在生物学和生物医学中,尤其在制药行业中,蛋白质的体外折叠复性是一个比较棘手的问题。最近利用层析方法复性成为研究的热点,本综述主要介绍层析复性方法的进展。 随着基因工程和生物技术的发展,蛋白质克隆与表达已经趋于成熟。在重组蛋白质生产的过程中,目标产物的异源
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
(cattle tick) Bm86I, S*, 1.5 g/L, SUC2[131, 132, 133]Cockroach allergen, Bla g 4S, 50 mg/L[134]Dermatophagoides pteronyssinus Der p 1S, 150 mg/L, α-MF, active563Diabrotica virgifera virgifera(corn rootworm) cysteine proteinaseS, α-MF, active357Drosophila
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