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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse C-C motif chemokine 21c (Ccl21c)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
Ccl21cUniprot No.:
P86793别名:
(6Ckine)(Beta-chemokine exodus-2)(Small-inducible cytokine A21c)(Thymus-derived chemotactic agent 4)(TCA4)种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
25.0 kDa表达区域:
24-133aa氨基酸序列:
SDGGGQDCCLKYSQKKIPYSIVRGYRKQEPSLGCPIPAILFLPRKHSKPELCANPEEGWVQNLMRRLDQPPAPGKQSPGCRKNRGTSKSGKKGKGSKGCKRTEQTQPSRG蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues." Lo J.C., Chin R.K., Lee Y., Kang H.S., Wang Y., Weinstock J.V., Banks T., Ware C.F., Franzoso G., Fu Y.X. J Clin Invest 112:1495-1505(2003)Function:
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文献和实验A chemokine-driven positive feedback loop organizes lymphoid follicles
Lymphoid follicles are B-cell-rich compartments of lymphoid organs that function as sites of B-cell antigen encounter and differentiation. CXC chemokine receptor-5 (CXCR5) is required for B-cell migration to splenic
Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
量的 ChIP 数据却非常具有挑战性。在每个关键步骤都有很多需要注意的细节。下面是 Active Motif ChIP 实验专家根据多年经验总结的每个步骤中的原理及一些需要注意的细节。第一步 染色质交联为什么要用甲醛交联?ChIP 技术主要是一种解码信息(目的蛋白在染色质上的分布)的方法。对于解码信息,首先就需要确保信息保存下来而不失真,ChIP 技术用到的最主要的信息保存方式是甲醛交联。甲醛交联也是 ChIP 实验非常关键的一个步骤。最早的 ChIP 技术是不用甲醛交联的[6],只是后来发现甲醛交联能更好的保存蛋白
T细胞, B细胞, 树突状细胞,活化的自然杀伤细胞 CCL20 SCYA20 exodus-1, MIP-3, LARC, ST38 (mouse) T细胞, B细胞 CCL21 SCYA21 exodus-2, SLC, 6-Ckine, TCA4, CK beta
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