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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fragment of human Transferrin.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX638745
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 抗体英文名:
Transferrin antibody [HL2429]
- 抗体名:
Transferrin 抗体 [HL2429]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Transferrin antibody [HL2429] detects secreted Transferrin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Transferrin stained by Transferrin antibody [HL2429] (GTX638745) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Mouse plasma and rat plasma (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [HL2429] (GTX638745) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [HL2429] (GTX638745) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transferrin antibody [HL2429] (GTX638745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献和实验[3H] Choline Labeling and TNF Treatment of HL-60 Cells
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 2 X 105 cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H
[3H] Choline Labeling and TNF Treatment of HL-60 Cells
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 2 X 105 cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H
Choline Labeling and TNF Treatment of HL-60 Cells
Choline Labeling and TNF Treatment of HL-60 Cells Contributor: Suprya Jayadev Date: Aug. 6, 1991 1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room
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