- ￥5300
- GeneTex
- 美国
- GTX57568
- 2025年07月10日
- WB, FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The clone AT4E6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human kLF6 protein.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX57568
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
KLF4
- 抗体英文名：
KLF4 antibody [AT4E6]
- 抗体名：
KLF4 抗体 [AT4E6]
- 规格：
100 μl
WB analysis of various samples using GTX57568 KLF4 antibody.
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:3000
FACS analysis of HeLa cells using GTX57568 KLF4 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg
FACS analysis of A549 cells using GTX57568 KLF4 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
(placing the cells on an ice-water bath between pulses).To eliminate foaming,place the tip of the horn near the bottom of the tube while touching the side with the horn .USE THE PLAIN WHITE TUBES. 10.Centrifuge at maximal setting at4℃for 10-15 min. 11
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