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文献和实验High‐Throughput RT‐PCR for Small‐Molecule Screening Assays
to measure the changes in gene expression due to perturbagens such as small molecules or siRNA. The availability of new instrumentation for liquid handling and real?time PCR analysis, as well as the commercial availability of start?to?finish kits for RT?PCR
Hints and precautions for the care, feeding and breeding of Neurospora
, protoperithecia may enlarge and become pigmented so as to resemble small perithecia. These "false perithecia" are devoid of beaks, asci, and ascospores, and unfertilized strains exhibiting them remain completely sterile. The strains can develop normal perithecia
clone in the Floor Shaker for 18 hours at 37 degrees C shaking at 350 rpm. 2.For BAC or Fosmid isolation for end sequencing: Using the Hydra96, inoculate 2 μl of the BACs or Fosmids from their glycerol plate stocks into either a 96 or 384
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