240ul 384 Deep Well "Diamond P

Gene Knockout Techniques(1) The Knockout Process

to save time and materials, but everything can be done in 96-well plates if you don?t have access to 384-well equipment. 10 ul reactions work fine in 96-well plates, and don?t require nearly as much Taq. To date we have done most steps with worms grown on solid

生物芯片实验手册（NIH）

to each well. Mix briefly. 9. Add 100 µl Neutralization buffer to each well. Vortex. 10. Transfer the contents of the deep wells to the waiting filter plates/receiving plate stacks using the wide bore pipette tips provided in the kits. 11