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文献和实验In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
just enough solution (20 µl) to cover the gel pieces (you only need to have the trypsin in the gel pieces to digest the proteins) After 15 minutes, check out the samples and add more trypsin solution if the solution is completely absorbed by the gel pieces
substances such as sucrose, glycerol etc. may be added or the experiment can be performed in a sieving medium such as a high concentration of polyacrylamide (PAA) gel. Increased viscosity will also affect the mobility (µ ) the mobility of the proteins
96 Well Mating Protocol platewise
% Glycerol (sterile) 10 ml 20% L-arabinose (filter sterile) 1 ml Carbenicillin at 100mg/ml (for pMSCV selection) AND antibiotic to select for your insert POUR YEGlu: 1 liter MilliQ water 5 g yeast extract 10 g NaCl autoclave and cool add 8 ml of 50
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