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文献和实验10 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly. 3. Run with constant voltage (120V) 4. Usual running time is about 1：40 F. Preparation of membrane 1. Cut a piece of PVDF membrane(Pall Corporation ). 2. Wet for about 30 min
precipitates. Discard the supernatant by decantation (Do not discard precipitates!). Rinse the precipitates twice with 70% ethanol. Transfer the precipitates to a 2 ml micro tube by decantation. Rinse the precipitates with 99.5% ethanol. Dry
2-D Polyacrylamide Gel Electrophoresis
of 0.5 M Tris-HCl,pH 6.9. 2.Vortex vigorously. 3.Add 10 ml of 20% SDS and 200 mg DTT. 4.Invert gently several times. 5.Add 15 ml glycerol. 6.Vortex vigorously (or attach tube to a rotator for 10-15 min). 7.Add Bromophenol Blue as indicator . F: 50 ml
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