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文献和实验Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
per 50ml 3-10 3-10 40% 5ul 25ul 250ul 4-7 4-6 40% 2.5ul 12.5ul 125ul 5-7 40% 2.5ul 12.5ul 125ul 3-6 3-5 20% 5ul 25ul 250ul 4-6 40% 2.5ul 12.5ul 125ul 5-8 5-8 40% 5ul 25ul 250ul 7-10 7-9 40% 2.5ul 12.5ul 125ul
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