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文献和实验An Ultra-fast method of DNA extraction from Neurospora
the need for large numbers of flasks when growing many cultures for DNA isolation. 1. Inoculate plastic Petri plates containing 25 ml of 1X Vogels, 2 sucrose, 0.04 Tween-80 (to prevent conidiation) (Zalokar, M. 1954. Arch. Biochem. Biophys. 50:71-80
Transformation Ultra-Competent E. coli
Transformation "Ultra-Competent" E. coli (Inoue Method) Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96 :23-28. Citation Abstract Procedure
of the tunica albuginea. Four to five of these pieces were immediately placed into a disposable disaggregator Medicon™ with 50 µm separator mesh (Becton Dickinson) plus 1 mL of ice-cold separation medium and processed for 50 s in the Medimachine System (Becton
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