相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州威佳科技有限公司
- 现货状态:
现货
- 库存:
500
- 保修期:
请咨询
- 规格:
请咨询
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CATHY'S NO BOIL YEAST EXTRACTS
CATHY'S NO BOIL YEAST EXTRACTS 1.Cells to mid log/ need ~ 5OD.Spin down and wash w/ 1ml H2O 2.Transfer to 1.5ml round bottom screw cap tube 3.add 200μl SDS sample buffer+ (5% BME,50mM NaF,1mM PMSF)-add to stock just before use; + 200μl glass
Co-staining with Thioflavin S & Carboxyfluorescein
Co-staining with Thioflavin S & Carboxyfluorescein 1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ [0.01g / 100 ml F12+] 2.) Filter the staining solution with a 0.22 µm Millipore filter
Kamps's Western Blotting Protocol
activity).We use 12-14 microCi (approx.10 microliters)125I-protein A in a volume of 30-35 ml blocking buffer.We reuse this solution 3 times without noticing a reduction in signal (see #6 in Comments). 9)Wash again as in step 7. Damp dry on Whatman 3MM
技术资料暂无技术资料 索取技术资料










