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- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IM2017
- 2025年12月13日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Mouse Tracheal Smooth Muscle Cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
C57
- 组织来源：
气管
- 相关疾病：
无
- 物种来源：
小鼠
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
气管
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化小鼠气管平滑肌细胞简介：
产品描述：哮喘是一种常见的气道慢性炎症性疾病，目前我国有约2000万人罹患哮喘。近些年，由于雾霾等空气质量与环境污染程度的日益恶劣，呼吸系统疾病也逐渐成为了全世界范围影响人类健康最严重的慢性疾病之一。据世界卫生组织预测，到2025年，世界上将会有 4 亿人患上哮喘。哮喘发 病 症 状 众 多，早 期 表 现 为 过 敏 性 炎症，后期由于气管平滑肌增生，阻塞气管，导致气道狭窄、气道高反应及气道重塑，患者出现胸闷、气喘等症状，严重时危及生命。因此，对哮喘的发病机理与治疗的研究是至关重要的。在对哮喘发病机制的研究中，气管平滑肌是针对气道阻塞性症状的主要研究目标之一，针对气管平滑肌细胞的形态学观察与细胞增殖速率的测量是在细胞生物学水平研究哮喘发病机制的常用手段。
产品货号：IM2017
产品类型: 原代细胞建立的永生化
传代能力: 30代左右
产品形态:梭形
培养基：永生化小鼠气管平滑肌专用完全培养基，产品编号：IM2017-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：1周左右货期
a-SMA抗体免疫荧光染色鉴定
Transcriptional regulation of VCAM-1 expression by tumor necrosis factor-alpha in human tracheal smooth muscle cells: involvement of MAPKs, NF-kappaB, p300, and histone acetylation.
Tumor necrosis factor- (TNF-) has been shown to induce the expression of adhesion molecules in airway resident cells and contribute to inflammatory responses. Here, the roles of mitogen-activated protein kinases (MAPKs) and NF-B in TNF--induced expression of vascular cell adhesion molecule (VCAM)-1 were investigated in human tracheal smooth muscle cells (HTSMCs). TNF--enhanced expression of VCAM-1 protein and mRNA as well as phosphorylation of p42/p44 MAPK, p38, and JNK were significantly attenuated by inhibitors of MEK1/2 (U0126), p38 (SB202190), and JNK (SP600125). Transfection with dominant negative mutants of MEK1/2, ERK1, ERK2, p38, and JNK attenuated TNF--induced VCAM-1 expression. Furthermore, TNF--induced VCAM-1 expression was significantly blocked by a selective NF-B inhibitor helenalin. TNF--stimulated translocation of NF-B into the nucleus and degradation of IB- was blocked by helenalin, but not by U0126, SB202190, or SP600125. VCAM-1 promoter activity was enhanced by TNF- in HTSMCs transfected with VCAM-1-Luc, which was inhibited
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
3K的下游靶点。 刚刚接触这方面的信号通路，望多指教，谢谢！ yhwangcams 呵呵，好多情况下得出的结论都可能源于不同的细胞系，不同的处理方法，等等。 各个信号通路间会有一些“交流”（crosstalk）。我的建议是先从Nature, Cell等上找一些大人物写的综述看看。基本了解后，再看相关细胞里（如平滑肌细胞等）的研究。自己能做出什么结果，只要可信，就可以得出相关结论。 本文由丁香园论坛提供，想了解更多有用的、有意
主要有两类：神经嵴干细胞(neuralcreststemcell，NC-SC)和中枢神经干细胞(CNS-SC)。NCSC为外周神经干细胞(PNS-SC)，既可发育为外周神经细胞、神经内分泌细胞和Schwann氏细胞，也能分化为色素细胞(pigmented cell)和平滑肌细胞等。NSC一般是指存在于脑部的中枢神经干细胞(CNS-SC)，其子代细胞能分化成为神经系统的大部分细胞。以往认为，中枢神经系统的神经元在出生前或出生后不久，就失去再生能力。但近年的一些研究表明，成年哺乳
胚胎成纤维细胞 NBLE 新生牛眼晶体上皮细胞 NBTF 新生牛眼Tenon's囊成纤维细胞 NIH3T3 小鼠胚胎成纤维细胞 P815 小鼠肥大细胞 PA12 小鼠成纤维细胞 RCBBF 兔角膜后基质层成纤维细胞 RCFBF 兔角膜前基质层成纤维细胞 RTE 大鼠气管上皮细胞 RYTF 兔眼Tenon's囊成纤维细胞 SF9 昆虫卵巢细胞 SMC 兔主动脉平滑肌细胞 Vero 猴肾细胞 WEHI-3 小鼠血液细胞 Y2
