- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IH1008
- 2026年09月02日
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- 文献和实验
- 技术资料
- 英文名：
Immortalized human umbilical artery smooth muscle cells
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
人源
- 组织来源：
脐动脉
- 相关疾病：
无
- 物种来源：
人源
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
脐动脉
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化脐动脉平滑肌细胞简介：
产品描述：脐带是胎儿和胎盘之间的连系结构。形状如绳索，表面光滑透明，内含结缔组织和一支脐静脉，一对脐动脉。近期的研究表明平滑肌细胞能表达钙离子通道，ICAM-1和VCAM-1。其中ICAM-1和VCAM-1的表达可能是造成血管壁炎症反应，并进一步造成血管疾病的原因 。因此，对血管平滑肌细胞的体外培养和研究可用来发现和确定新的血管疾病的靶向治疗方法。
产品货号：IH1008
产品类型: 原代细胞永生化
传代能力: 30代左右
产品形态: 梭形
培养基：永生化人脐动脉平滑肌细胞专用完全培养基，产品编号：IH1008-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：常温T25方瓶运输
货期：1周左右货期
a-SMA抗体免疫荧光染色鉴定
[Biological characteristics of human umbilical artery smooth muscle cells cultured in vitro and the preestablishment of immortalized cell line].
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
普诺赛 hUC-MSCs原代分离与鉴定全流程详解（组织贴块法）
细胞活率和纯度。 ②剥除脐动脉和脐静脉 将脐带剪成约6 cm的小段，纵向剪开后可见两条脐动脉和一条脐静脉。使用无菌器械仔细剥离血管，尽量保持血管组织完整和剔除干净（图1）。 注：血管组织中包含平滑肌细胞、成纤维细胞等细胞，如果剔除不彻底，后续培养过程中可能出现非目标细胞混杂，与hUC-MSCs竞争营养，甚至抑制hUC-MSCs的贴壁和增殖。 图1. 剔除干净血管后的脐带组织 ③去除羊膜 用无菌镊子剥离去除脐带外层羊膜组织，保留内部华通氏胶。 注：zui大程度降低非目标组织混入
glutamine-pyruvate-phenol-red-no-hepes.html DMEM (Dulbecco’s Modified Eagle Medium) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 Hela、293、Cos-7、PC-12 等细胞系，以及原代成纤维细胞、神经元、神经胶质细胞、人脐带静脉内皮细胞、平滑肌细胞等。 青霉/链霉素双抗溶液 www.medchemexpress.cn/inhibitor-kit/penicillin-streptomycin-(100%C3
glutamine-pyruvate-phenol-red-no-hepes.html DMEM (HY-K3001) 是广泛使用的基础培养基，适于培养多种哺乳动物细胞，包括 Hela、293、Cos-7、PC-12 等细胞系，以及原代成纤维细胞、神经元、神经胶质细胞、人脐带静脉内皮细胞、平滑肌细胞等。 DMEM/F-12 (1:1) 培养基，含 L-谷氨酰胺、酚红和 HEPES www.medchemexpress.cn/inhibitor-kit/dmem-f-12-1-1-l-glutamine-phenol-red
技术资料
