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文献和实验C. elegans whole mount immunohistochemistry with Bouin's Fixative
C. elegans whole mount immunohistochemistry with Bouin's Fixative I have developed a fixation protocol which provides an alternative set of conditions to test antisera that work poorly on paraformaldehyde
Protocols for the preparation of tumour cells for s.c. injections in mice
) at 37 °C and 6% CO2 . The tumour models used in our laboratory are obtained by subcutaneous (s.c.) injection of the following cell lines in nude mice: C51 mouse colon adenocarcinoma FE-8 rat transformed fibroblasts F9 murine teratocarcinoma
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
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