- ￥500
- BioToolomics
- 英国
- 390201-50ML
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
不可逆硫醇耦联磁性琼脂糖介质4HF
产品名称：不可逆硫醇耦联磁性琼脂糖介质4HF英文名称：Irreversible Thiol-Coupling SepFast MAG
Product Introduction:
Irreversible Thiol-coupling SepFast MAG is used to make magnetic adsorbents to immobilize thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or special equipment is required.
Irreversible Thiol-coupling SepFast MAG medium forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm (12 atoms) that facilitates much improved ligand accessibility. This pre-activated magnetic agarose resin can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Product Properties:
There is a choice of two different activated base matrices that suit ligands of various molecular weights.
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Irreversible Thiol-Coupling SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.
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Irreversible Thiol-Coupling SepFast MAG 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity and allows harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm (12 atoms). It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
作用，保持质粒DNA的稳定性，另外SolutionI中的Tris起着稳定pH的作用，葡萄糖起着缓冲防止gDNA断裂的作用。SolutionII是非常关键的一步，SDS破坏胞膜，释放菌体内容物，同时也能使绝大多数的蛋白质和脂肪变性并溶解，NaOH使质粒DNA和基因组DNA都变性，而质粒DNA由于有特有的拓扑超螺旋结构，可以在下一步中自然复性，而基因组DNA则永久变性，在这一步中注意事项是在混匀过程中动作必须轻柔，原因是防止局部pH大于13而导致质粒DNA不可逆变性和粗暴操作导致的基因组断裂，局部高浓度
室温时平均热运动能(2.5kj/mol)。如果两个分子表面几何形态互补，由于许多原子协同作用，范德华力就能成为分子间有效引力。范德华力对生物多层次结构的形成和分子的相互识别与结合有重要意义。 (四)、荷电基团相互作用 荷电基团相互作用，包括正负荷电基团间的引力，常称为盐键(salt bond)和同性荷电基团间的斥力。力的大小与荷电量成正比，与荷电基团间的距离平方成反比，还与介质的极性有关。介质的极性对荷电基团相互作用有屏蔽效应，介质的极性越小，荷电基团相互作用越强。例如，-COO
