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- BioToolomics
- 英国
- 330101-5ML
- 2025年07月12日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
NHS活化琼脂糖凝胶4HF
产品名称：NHS活化琼脂糖凝胶4HF
英文名称：NHS-activated SepFast 4HF
Product Introduction:
NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in 100% isopropanol.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
磁珠偶联链霉亲和素（SA）和抗体的实验方法及偶联实验过程中的一些注意事项。 一、羧基磁珠偶联链霉亲和素（SA）和抗体的原理 EDC通过与磁珠上的羧基进行反应，形成一种促发剂——不稳定的脲衍生物，NHS通过形成更稳定的酯而增强碳二亚胺交联产物的稳定性。交联过程中EDC、NHS不进入最终产物中，而是转变成水溶性的脲衍生物。 二、羧基磁珠偶联链霉亲和素（SA） 1. 偶联条件 1.1. 活化剂：EDC和NHS 1.2. 反应温度：37℃环境 1.3. 反应时间：
标记抗体包含清洗-活化-清洗-偶联-封闭-清洗-复溶保存7个步骤，其中活化、偶联和封闭是免疫标记的重要环节。以下我们会介绍微球在免疫标记中活化、偶联和封闭实验过程中的一些注意事项。 微球免疫标记抗体步骤 01. 活化 微球免疫标记常用的活化剂有EDC和NHS。EDC是极易溶于水的碳二亚胺，在酰胺合成中，用作羧基的活化试剂，使用时的PH范围一般为4.0-6.0，常和NHS连用，以提高偶联效率。在活化微球上的羧基时，微球跟活化剂的质量比，EDC跟NHS的质量比都有一个合适的比例，过少会导致活化不充分，偶联
分子在纯化时若无商品化的亲和填料可供选择，也可以利用预活化填料，按照说明书介绍的流程，自行偶联生物分子到填料上，形成独有的亲和填料，来分离纯化其对应的生物分子，如酶/底物、抗原/抗体等。如需要纯化某种单抗，可以将相应的抗原偶联到预活化填料NHS activated Sepharose 4FF或CNBr activated Sepharose 4FF上，然后通过亲和层析实验，就可以实现抗体的高纯度纯化。偶联配基时，需要考虑活化基团是否与填料匹配，以及填料本身是否带有间隔臂。如果偶联的配基是蛋白
