- ￥500
- BioToolomics
- 英国
- 390204-5ML
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
不可逆硫醇耦联磁性琼脂糖凝胶6HF
产品名称：不可逆硫醇耦联磁性琼脂糖凝胶6HF英文名称：Irreversible Thiol-Coupling SepFast MAG
Product Introduction:
Irreversible Thiol-coupling SepFast MAG is used to make magnetic adsorbents to immobilize thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or special equipment is required.
Irreversible Thiol-coupling SepFast MAG medium forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm (12 atoms) that facilitates much improved ligand accessibility. This pre-activated magnetic agarose resin can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Product Properties:
There is a choice of two different activated base matrices that suit ligands of various molecular weights.
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Irreversible Thiol-Coupling SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.
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Irreversible Thiol-Coupling SepFast MAG 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity and allows harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm (12 atoms). It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
网状结构。以使被分离的生物分子能够均匀、稳定的通透，并充分与配体结合。基质的孔径过小会增加基质的排阻效应，使被分离物与配体结合的机率下降，降低亲和层析的吸附容量。所以一般来说，多选择较大孔径的基质，以使待分离物有充分的空间与配体结合。4.基质本身与样品中的各个组分均没有明显的非特异性吸附，不影响配体与待分离物的结合。基质应具有较好的亲水性，以使生物分子易于靠近并与配体作用。一般纤维素以及交联葡聚糖、琼脂糖、聚丙烯酰胺、多孔玻璃珠等用于凝胶排阻层析的凝胶都可以作为亲和层析的基质，其中以琼脂糖凝胶
作用，保持质粒DNA的稳定性，另外SolutionI中的Tris起着稳定pH的作用，葡萄糖起着缓冲防止gDNA断裂的作用。SolutionII是非常关键的一步，SDS破坏胞膜，释放菌体内容物，同时也能使绝大多数的蛋白质和脂肪变性并溶解，NaOH使质粒DNA和基因组DNA都变性，而质粒DNA由于有特有的拓扑超螺旋结构，可以在下一步中自然复性，而基因组DNA则永久变性，在这一步中注意事项是在混匀过程中动作必须轻柔，原因是防止局部pH大于13而导致质粒DNA不可逆变性和粗暴操作导致的基因组断裂，局部高浓度
能量的驱动。在原发主动转运过程中能源可以是光，ATP或电子传递；而第二级主动转运是在离子浓度梯度下进行的。 协同运输（contransport）：两种不同溶质的跨膜的耦联转运。可以通过一个转运蛋白进行同一方向（同向转运）或反方向（反向转运）转运。 胞吞（信用）（endocytosis）：物质被质膜吞入并以膜衍生出的脂囊泡形成（物质在囊泡内）被带入到细胞内的过程。 第四章 蛋白质 提要 一. 概念 简单蛋白、结合蛋白、基本氨基酸、等电点、甲醛
