- ￥500
- BioToolomics
- 英国
- 310201-10G
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
1G;10G;100G
产品名称：CNBr活化磁性琼脂糖介质4HF
英文名称：CNBr-activated SepFast MAG
Product Introduction:
CNBr activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.
CNBr-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
The application areas cover immobilization of proteins, peptides and nucleic acids.
Product Properties:
CNBr activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible so that low pH and certain organic solvents of high concentrations can be applied.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized. CNBr-activated SepFast MAG media is supplied as a freeze-dried powder stabilized with additives.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
亲和层析的实验操作和一般柱层析类似，都包括：装柱、上样、洗脱、样品收集、结果处理等步骤。溴化氰活化琼脂糖用于配体固相化是实验室亲和层析最常用的技术之一，该方法简便廉价，并广泛应用于蛋白质、核酸、凝集素等。下面以此为例介绍亲和层析的实验方法：试剂与配制1.Sepharose 4B2.CNBr（剧毒）3.抗原或抗体4.1Mol/L NaHCO3 取NaHCO3 84.01g加水至1 000ml。5.0.1Mol/L NaHCO36.2Mol/L NaOH7.0.01Mol/L pH7.4 PBS
粒子的表面(或孔内)即可制备亲和吸附介质，该固体粒子通常称为配基的载体。因此，亲和吸附介质又称亲和载体。作为载体的固体粒子应满足下列要求:(1)具有亲水性多孔结构，无非特异性吸附，比表面积大;(2)物理和化学稳定性高，有较高的机械强度，使用寿命长;(3)含有可活化的反应基团，用于亲和配基的固定化;(4)粒径均一的球形粒子。常用的亲和层析载体有:琼脂糖凝胶和交联琼脂糖凝胶、聚丙烯酰胺凝胶、葡聚糖凝胶、聚丙烯酰胺—琼脂糖凝胶、纤维素、多孔玻璃等。其它用于载体的物质还有:聚苯乙烯、淀粉珠、壳聚糖、硅胶
过配体为FMN的亲和胶？亲和的填料合成过20来种，你是希望用它来纯化某种脱氢酶吗，我看FMN可偶联的有限，倒是FAD有活泼的氨基好偶联，何况它们几乎是同一个辅酶，你是不是考虑把FAD连上去。当然FMN的结构上也有个仲胺，可以偶联到带长手臂的环氧活化的填料上，而溴化氰活化或别的活化的介质都不大好，因此我觉得这个没有什么问题。此外如果是以FMN为辅酶的，那我还可以建议你试试蓝色琼脂糖凝胶，因为它的配基的结构和FMN的三个环很相似，它能纯化不少脱氢酶和激酶。我已经给你发了相关一些偶联的材料，请查收，现在
