- ￥500
- BioToolomics
- 英国
- 390101-5ML
- 2025年07月15日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
不可逆硫醇耦联琼脂糖凝胶4HF
产品名称：不可逆硫醇耦联琼脂糖凝胶4HF英文名称：Irreversible Thiol-coupling SepFast
Product Introduction:
Irreversible Thiol-coupling SepFast can be used for the specific immobilization of thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or equipment is required.
Irreversible Thiol-coupling SepFast media forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm that facilitates much improved ligand accessibility. This pre-activated agarose based resin can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
There is a choice of two different activated base matrices that suit ligands of various molecular weights.
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Irreversible Thiol-Coupling SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
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Irreversible Thiol-Coupling SepFast 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm. It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
网状结构。以使被分离的生物分子能够均匀、稳定的通透，并充分与配体结合。基质的孔径过小会增加基质的排阻效应，使被分离物与配体结合的机率下降，降低亲和层析的吸附容量。所以一般来说，多选择较大孔径的基质，以使待分离物有充分的空间与配体结合。4.基质本身与样品中的各个组分均没有明显的非特异性吸附，不影响配体与待分离物的结合。基质应具有较好的亲水性，以使生物分子易于靠近并与配体作用。一般纤维素以及交联葡聚糖、琼脂糖、聚丙烯酰胺、多孔玻璃珠等用于凝胶排阻层析的凝胶都可以作为亲和层析的基质，其中以琼脂糖凝胶
能量的驱动。在原发主动转运过程中能源可以是光，ATP或电子传递；而第二级主动转运是在离子浓度梯度下进行的。 协同运输（contransport）：两种不同溶质的跨膜的耦联转运。可以通过一个转运蛋白进行同一方向（同向转运）或反方向（反向转运）转运。 胞吞（信用）（endocytosis）：物质被质膜吞入并以膜衍生出的脂囊泡形成（物质在囊泡内）被带入到细胞内的过程。 第四章 蛋白质 提要 一. 概念 简单蛋白、结合蛋白、基本氨基酸、等电点、甲醛
