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- 库存:
大量
- 供应商:
北京百奥创新科技有限公司
- 规格:
5mL;50mL;1L
胺基活化磁性琼脂糖磁珠
Product Introduction:
Amine-activated SepFast MAG is used to make magnetic chromatography adsorbents. It forms chemically stable amide linkages with ligands containing carboxyl groups. It has a long spacer (9 atoms). After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Amine-activated agarose can be used for the immobilization of carboxyl containing, or aldehyde containing molecules. The coupling chemistry is carbodiimide-based or reduced amination based and is a successful and well-documented technique.

Product Properties:
Amine-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible, so that low pH and certain organic solvents of high concentrations can be applied.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
后,将EP管置于磁性分离架上,去除上清,加入封闭液,此时,磁珠的终浓度为5mg/mL,同样置于37℃环境旋转混匀反应1h。 2.5. 封闭完成后,重复2.2步骤,最后将磁珠用保存液保存,此时磁珠的浓度可自定。 三、羧基磁性微球偶联抗体 1. 偶联条件 1.1. 活化剂:EDC和NHS 1.2. 反应温度:37℃环境 1.3. 反应时间: 1.3.1.活化时间:30min 1.3.2.偶联时间:2h
的不同可以将免疫磁性细胞分选分为直接分选法和间接分选法。 直接分选法是指将抗体或者亲和配体直接包被在磁性颗粒上,形成免疫磁性复合微粒,将其与多细胞悬液混合孵育之后,目的细胞会特异性的结合在免疫磁珠上,在外加磁场作用下,带有磁珠的目的细胞会滞留在磁场中,而非目的细胞则会被去除。 间接分选法分选细胞引入了二抗,即将目的细胞首先与对应的一抗孵育,激活细胞,之后清洗出去未结合的一抗,然后加入预先包被有二抗的磁性颗粒与活化后的细胞孵育,一抗与二抗之间的相互作用会导致磁粒结合在目的细胞上,同样在磁场作用下滞留
技术丨化学发光免疫分析中羧基与Tosyl磁珠包被抗体方法及注意事项
化学发光免疫分析与磁珠 化学发光免疫分析(chemiluminescence immunoassay,CLIA),是具有高灵敏度的化学发光测定技术与高特异性的免疫反应相结合,通过抗原抗体的免疫反应检测各项标志物的免疫分析技术。该技术广泛用于传染病,心脏疾病,肿瘤,妊娠检测等领域。是现阶段体外诊断领域最主流的技术和趋势。磁性微粒(磁珠)是化学发光免疫分析中的一种重要原料。常用磁珠主要是粒径1-3um,修饰了羧基、甲苯磺酰基或者链霉亲和素的免疫磁珠。今天小编就以JSR磁珠为例,为大家介绍一下






