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- BioToolomics
- 英国
- 410204-50ML
- 2025年07月12日
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大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
环氧活化磁性琼脂糖介质6HF
产品名称：环氧活化磁性琼脂糖介质6HF英文名称：Epoxy activated SepFast MAG
Product Introduction:
Epoxy-activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.
Epoxy-activated agarose can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary aminecontaining or thiol-containing molecules. Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable.
Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
There is a choice of two different Epoxy activated magnetic resins that suits various ligands and applications.
• Epoxy activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It is useful for the coupling of protein molecules.
• Epoxy activated SepFast MAG 6HF is made of highly cross-linked 6% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It has high levels of epoxy groups for applications that require high ligand densities. It is useful for the coupling of small molecules or peptides etc.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules. The material is activated through attaching a hydrophilic carbon chain with epoxy group in the end.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
的浓度降低点，这也是个办法。 2)请问有没有合成过配体为FMN的亲和胶? 亲和的填料合成过20来种，你是希望用它来纯化某种脱氢酶吗，我看FMN可偶联的有限，倒是FAD有活泼的氨基好偶联，何况它们几乎是同一个辅酶，你是不是考虑把FAD连上去。当然FMN的结构上也有个仲胺，可以偶联到带长手臂的环氧活化的填料上，而溴化氰活化或别的活化的介质都不大好，因此我觉得这个没有什么问题。 此外如果是以FMN为辅酶的，那我还可以建议你试试蓝色琼脂糖凝胶，因为它的配基的结构和FMN
过配体为FMN的亲和胶？亲和的填料合成过20来种，你是希望用它来纯化某种脱氢酶吗，我看FMN可偶联的有限，倒是FAD有活泼的氨基好偶联，何况它们几乎是同一个辅酶，你是不是考虑把FAD连上去。当然FMN的结构上也有个仲胺，可以偶联到带长手臂的环氧活化的填料上，而溴化氰活化或别的活化的介质都不大好，因此我觉得这个没有什么问题。此外如果是以FMN为辅酶的，那我还可以建议你试试蓝色琼脂糖凝胶，因为它的配基的结构和FMN的三个环很相似，它能纯化不少脱氢酶和激酶。我已经给你发了相关一些偶联的材料，请查收，现在
相关专题 蛋白纯化技术专题 各种His.Bind基质的产品如何选择：本文介绍带Ni NTA琼脂 糖、无Ni IDA琼 脂糖、带Ni的IDA琼脂糖预装柱、无Ni IDA触须甲酰化、带Ni的IDA纤维素预装筒、带Ni的IDA磁性琼脂糖……等His.Bind基质的特点和应用。 His.Bind基质选择指南：与任何纯化介质一样，His.Bind树脂在接近结合载量时使用，可获得最干净的分离
