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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
carried over through protein-protein interactions. The protocol includes purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP
Optimising Yeast as a Host for Recombinant Protein Production (Review)
typically allows the isolation of the pure protein in quantities much higher than those found in the protein’s native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to the native human source cells of many proteins
Protein Secondary Structure Prediction
Structure and the Principles of Protein Conformation. Plenum, New York. Fermi, G. 1975. Three‐dimensional Fourier synthesis of human deoxyhaemoglobin at 2.5 angstroms resolution
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