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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C
- 保质期:
1
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)
- 抗体英文名:
LexA | LexA repressor
- 抗体名:
LexA | LexA阻遏物
- 规格:
50ul
关键词:LexA | LexA repressor,LexA | LexA阻遏物,LexA | LexA阻遏物
产品名称:LexA | LexA阻遏物-LexA | LexA repressor
产品货号:AS21-4541
产品规格:50ul
应用类型:Chromatin immunoprecipitation (ChIP), Immunolocalisation (IL), Western blot (WB)
保存建议:LexA | LexA阻遏物-LexA | LexA repressorStore at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
背景资料:LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.Alternative names: exrA, spr, tsl, umuA
点击:LexA | LexA阻遏物-LexA | LexA repressor查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询艾美捷科技有限公司。更多Agrisera公司特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验Two-hybrid analysis of genetic
therein)uses the E.coli protein LexA as the DNA-binding domain and a protein encoded by random E.coli sequences,the B42 "acid blob",as the transcription activation domain.Both proteins are expressed from multicopy (2µ)plasmids; the LexA fusion,or bait
基酸(GKPIPNPLLGLDST)。水泡性口炎病毒糖蛋白(VSV-G)VSV-G氨基酸序列为(YTDIEMNRLGK)。酵母双杂交标签:B42,GAL4,LexA,VP16这些标签用于酵母双杂交系统中的蛋白质相互作用,可作为 DNA 结合(GAL4,LexA)结构域;转录激活因子(B42,GAL4,VP16)
酵母双杂交实验技币和其他双成分系统 酵母 双杂交系统是以真核细胞转录激活因子的结构和活性特点为基础。真核细胞转录激活因子的结构都基本相似,由一个DNA结合结构域(BD)和一个转录激活结构域(AD)组成,如酵母蛋白GAL4,这两个结构域相互独立但功能上有相互依赖,它们之间只有通过某种方式结合在一起才有完整的转录激活因子活性。根据这一特点建立了酵母双杂交系统。具体实验步骤如下。 1.第一阶段(诱饵―LexA融合蛋白的鉴定)
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