Anti-E.coli lexA/LexA represso
r Nanobody (SAA1458), 抗E.coli lexA/LexA repressor纳米抗体 (SAA1458)- ￥1338
- abinscience
- France
- JN826013
- 2026年03月03日
- Escherichia coli M056
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
VHH-8His-Cys-tag
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4.
- 克隆性：
Monoclonal
- 适应物种：
Escherichia coli M056
- 保质期：
1 year
- 抗原来源：
Alpaca
- 目录编号：
JN826013
- 级别：
科研级别
- 库存：
999
- 供应商：
abinscience
- 浓度：
1mg/ml
- 抗体英文名：
Anti-E.coli lexA/LexA repressor Nanobody (SAA1458)
- 抗体名：
Anti-E.coli lexA/LexA repressor Nanobody (SAA1458)
- 规格：
50ug
| Product name | Anti-E.coli lexA/LexA repressor Nanobody (SAA1458) |
|---|---|
| Catalog No. | JN826013 |
| Host species | Alpaca |
| Specificity | \ |
| Conjugation | \ |
| Species reactivity | Escherichia coli M056 |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | VHH-8His-Cys-tag |
| Applications | ELISA |
| Target | LexA repressor, lexA |
| Purification | Purified by Nickel column. |
| Accession | A0A1X3HXW2 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | SAA1458 |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Two-hybrid analysis of genetic
therein)uses the E.coli protein LexA as the DNA-binding domain and a protein encoded by random E.coli sequences,the B42 "acid blob",as the transcription activation domain.Both proteins are expressed from multicopy (2µ)plasmids; the LexA fusion,or bait
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
The two-hybrid system developed in the Brent lab (the interaction trap) uses the E.coli protein LexA as the DNA-binding domain and a protein encoded by random E. coli sequences, the B42 "acid blob", as the transcription activation domain. Both proteins
组成的LexA蛋白)与目标蛋白(钓饵，Bait)的融合蛋白，该融合体的表达受酵母强启动子ADH1的调控，选择与报告基因的操纵子LexA×8结合。 质粒pB42AD的筛选标志为TRP1，在其供外源基因插入的多克隆位点(EcoR I与Xho I)上游，含有SV40核定位(SV40 nuclear localization)、HA(血凝素)及AD(来自于E.coli 的88个氨基酸残基组成的B42蛋白)等几种编码序列，共同组成可以启动报告基因转录表达的激活成份。 在酵母EGY48的基因
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