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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of human H4C1
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid freeze / thaw cycles.
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
H4C1
- 规格:
100ul
产品简介
| 靶标 | H4C1 |
| 别名 | H4/p;Histone H4 |
| 宿主 | Rabbit |
| 稀释比例 | WB 1:200-1000. |
| Gene ID | 121504 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Swiss Prot | P62805 |
| 蛋白分子量 | 11kDa |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 亚细胞定位 | Nucleus |
| 免疫原 | Recombinant protein of human H4C1 |
| 亚型 | IgG |
| 纯化方法 | Affinity purification |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. |
| Public Immunogen Range | 1-104aa |
| 克隆类型 | Polyclonal Antibody |
| 应用 | WB |
| 规格 | 50ul 100ul |
备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with H4C1 antibody diluted at 1:500;Lane:HeLa,MCF7,NIH3T3,C2C12,COS7,Rat spleen
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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