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文献和实验Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
were grown with feeder support and the medium was supplemented with basic fibroblast growth factor (FGF2) and platelet-derived growth factor (PDGF)-AB. A highly enriched MSC culture could be obtained by repeated passaging by trypsinization. The enriched MSC
‐767. Kuhn, H.G., Winkler, J., Kempermann, G., Thal, L.J., and Gage, F.H. 1997. Epidermal growth factor and fibroblast growth factor‐2 have different effects on neural
the number of colonies counted by the dilution factor to obtain the population density of the original broth culture. Notes A growth curve can be established by repeating this procedure every two hours. Since the number of bacteria can be large
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