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Overview
VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs). This hydrogel system can be used to make hydrogel cell beads for MSC scale-up. Microcarriers are not required for MSC scale-up.
VitroGel MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.
3D cell culture process in 20 min – “Just add cells”
VitroGel MSC is ready-to-use. Just mix with your cells.
MSC 3D scale-up process in 20 min – Hydrogel-cell bead formation
VitroGel MSC is ready-to-use. Just mix with your cells and add as droplets.
Specifications
| Formulation | Xeno-free, functional hydrogel |
| Use | 3D cell culture, 2D hydrogel coating, hydrogel-cell bead formation |
| Operation | Ready-to-use at room temperature |
| Biocompatibility | Biocompatible, safe for animal studies |
| Injection | Injectable hydrogel for in vivo studies and lab automation |
| Cell Harvesting | Easy cell recovery using VitroGel Cell Recovery Solution |
| pH | Neutral |
| Storage | Store at 2-8°C. Ships at ambient temperature |
| Sizes | 10 mL and 2 mL |
| Usage | 300 uses (10 mL), 60 uses (2mL) |
Data and References
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文献和实验脂肪（HA）来源的干细胞扩增并分化为脂肪细胞或骨细胞。 间充质干细胞方案 人骨髓间充质干细胞的扩增与分化 人脂肪间充质干细胞（SCC038）可以先在组织培养塑料上扩增，然后接种在TrueGel3D®HTS水凝胶板（TRUE-HTS1，TRUE-HTS10）中。或者，细胞可以解冻并直接接种在TrueGel3D®HTS水凝胶板中。在将细胞接种到TrueGel3D®HTS水凝胶板之前，确保细胞处于高活力状态。 培养基的准备: 2D MSC扩增培养基: 向α-MEM (M4526)中添加10
®悬滴板中生长可以自行生成3D细胞外基质，这样的细胞球体与体内组织更为相似，”Schrader说。“该产品也可以实现与其他类型的细胞共培养，如内皮细胞、基质细胞和上皮细胞等。” InfiniteBio公司也有供细胞球体研究的3D培养系统，SCIVAX 3D Nano Culture® Plate。该产品的孔底部具有纳米印迹nano imprinting技术形成的粘附表面，细胞球体就在该表面上形成。“我们系统的独特性在于，细胞球体在维持三维结构的同时是粘附在培养板上的，其优势在于操作更
E-Z 96® M13 Isolation Vacuum Manifold Protocol
1/5 volume of MPG Buffer (200μl MPG per 1 mL culture) to the M13 supernatant and mix by vortexing. Incubate at room temperature for 15 minutes. 5. Assemble the vacuum manifold by following the manufacturer’s instructions. If using Vac-03 vacuum manifold
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