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文献和实验as described earlier (25 ). Briefly, mouse IgG was non-covalently immobilized on the sample slide, or rabbit IgG on the control slide. Slides were covered with 20 μg/ml IgG solutions in 50 mM Na-phosphate buffer (pH 7.3) and incubated at room temperature
is to determine the cell density of the culture. Cell cultures always have some dead cells; the viable and non-viable cells can be distinguished with the use of trypan blue dye and a hemacytometer. Living cells will not take up the dye while dead cells do.
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states such as cancer and fibrosis. In this study, we report
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