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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
can be made. For the introduction of randomized X positions in the peptide sequences, take the appropriate Fmoc-AA-mix stock solution from the freezer, bring to room temperature, and activate by adding DIC (1.5 µl per 100 µl-vial
represents most probably a series of products with elongated CAGs. In lane 2 the faint band of about 4.4 kb (AA = additional amplicons) may be caused by the linear amplification of the target circular clones which in this case have been not digested
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
. Each primer in the pool also contains a single 5' nondegeneratenucleotide sequence derived from a codon consensus across the aligned aminoacid sequencesflanking the conserved motif. During the initial PCR amplification cycles, the degenerate core
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