硝基酪氨酸/硝基化酪氨酸抗体

 


 
产品属性：

产品名称	硝基酪氨酸/硝基化酪氨酸抗体
浓度	1mg/ml
型号	EY-01K2736

产品名称：硝基酪氨酸/硝基化酪氨酸抗体
英文名称：3-Nitrotyrosine
英文别名:NO tyrosine; nTyr; Nitrotyrosine; nitro-Tyrosine; nitro Tyrosine.
中文别名: 酪氨酸硝基化；
交叉反应: Human, Mouse, Rat
标记: Unconjugated
抗体来源: Rabbit
抗体类型: Polyclonal
免疫原: KLH conjugated Nitrotyrosine
蛋白细胞定位: 细胞浆
纯化方法: Affinity purified by Protein A
亚型: IgG
性状: Liquid
浓度: 1mg/ml
储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
背景资料: Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
抗体分子标记技术：
（一）原理
当应用化学氧化法时(NaI)遇强氧化剂,离子被氧化为分子,所生成的自由分子可与某些基团进行卤化反应。蛋白质分子可进行卤化反应的基团主要为酪氨酸残基,某些组氨酸残基也可能进行化。在应用胺T( Chloramine T)法的实验中,所用的氧化剂(1,3,4,6- tetrachloro-3a,6adipheny l-glucoluril)强挥发性的有机溶剂中。该溶剂加入试管后,先让其挥发(即让氧化剂将试管包被),然后把Na125I和蛋白质液加入包被好的试管中,反应完成即将混合液移入他管终止反应。
（二）准备
1.试剂
经亲和层析纯化的多克隆抗体或单克隆抗体；0.5mol/L磷酸钠缓冲液,pH7.5；无载体的Na125I3.7GBq/ml(100mCi/ml)的NaOH液；100g/L三醋酸；70%乙醇；新鲜制备的含2mg/m1络胺T的0.5mol/L磷酸钠缓冲液(pH7.5）；胺T反应终止缓冲液；
2.仪器
凝胶过滤柱；￥-记数器；玻璃纤维滤。
（三）操作要点
1.用胺T法进行化,也可在大约pH7.0的缓冲液中进行；
2.如果氧化反应损伤蛋白质，可将抗体与异硫酸荧光素(FITC)偶联)胺T量减少到0.02mg/m，并将偏重亚硫酸液的浓度降到0.024mg/ml；
3. Iodogen-包被的试管可在干燥器中,于室温下保存多年；
4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天）；
5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低；
6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。
抗体制备过程：
1.材料与试剂
  a．提取的动物 Ig
  b．弗氏完全佐剂和弗氏不完全佐剂
  c．青霉素和链霉素
  d．实验动物 兔
  e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
Anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) /FITC 荧光素标记磷酸化p21激活激酶1/2抗体IgGMulti-class antibodies规格： 0.2ml
Goat Anti-rat IgG 羊抗大鼠IgG (亲和层析纯化)Multi-class antibodies规格： 1mg
G/鸟苷酸结合抗体 Anti-G Protein 0.1ml
Donkey Anti-Guinea pig IgG/Bio 标记的驴抗豚鼠IgG 0.1ml
phospho-FLNC (2233)  磷酸化细丝2抗体 0.1ml
Rhesus antibody Rh Phospho-SRF (Ser77) 磷酸化生长激素释放抗体(清应答) 规格 0.1ml
Goat Anti-rat IgG 羊抗大鼠IgG (亲和层析纯化)Multi-class antibodies规格： 1mg
AT2R-Ab(Human Angiotensin II Receptor 2 antibody) ELISA Kit 人管紧张素Ⅱ受体2抗体Multi-class antibodies规格： 48T
Anti-IFNGR1/CD119 干扰素-gamma受体1抗体Multi-class antibodies规格： 0.2ml
Rhesus antibody Rh MAOA 单氨氧化酶A抗体 规格 0.1ml
MASP(Human mannan binding lectin associated serine protease) ELISA Kit 人甘露聚糖结合凝集素相关丝酸酶 96T
RPE65  视网膜色素上皮特异性65抗体 0.2ml
Phospho-Beta Catenin (Ser45)  磷酸化β 连环素抗体 0.1ml
Anti-IFNGR1/CD119 干扰素-gamma受体1抗体Multi-class antibodies规格： 0.2ml
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Rhesus antibody Rh CRF/CRH 促肾上腺皮质激素释放抗体 规格 0.1ml
EpCAM 上皮粘附分子/表皮粘附分子抗原 0.5mg
KEAP1  胞质接头Keap1 0.1ml
Rhesus antibody Rh Unc18-3 突触囊泡融合Unc18-3抗体 规格 0.2ml
Anti-EphA4/FITC 荧光素标记抗激酶A4抗体IgGMulti-class antibodies规格： 0.2ml
ASMA(Mouse Anti-Smooth Muscle Antibody) ELISA Kit 小鼠抗平滑肌抗体 96T
ODZ3  固生3抗体 0.2ml
Rhesus antibody Rh GRAMD3/NS3TP2 丙型毒-NS3反转录激活2抗体 规格 0.2ml
Human Thrombopoietin,TPO ELISA kit 人小板生成素Multi-class antibodies规格： 48T
Brs3  蛙皮素受体3抗体 0.1ml
Anti-SCG3/SgIII 分泌粒3抗体Multi-class antibodies规格： 0.2ml
硝基酪氨酸/硝基化酪氨酸抗体Anti-SH2B2 Antibody心肌营养素1
Anti-SH2D5 Antibody抗核抗体
Anti-SH2D2A Antibody维生素D3
Anti-SH3GLB2 Antibody抗肝肾微粒体抗体
Anti-SH3BGR Antibody抗α-胞衬抗体IgG/IgA
Anti-SH2D5 Antibody抗α干扰素抗体
Anti-SH3GLB2 Antibody抗凝酶Ⅲ抗体
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